Coding

Part:BBa_K2933021:Design

Designed by: Weisi Wang   Group: iGEM19_TJUSLS_China   (2019-09-06)


subclass B1 metallo-beta-lactamase Fla.103, codon optimized in E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 76
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 558
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized in E. coli.


Source

Flavobacterium sp. 103

References